Enhancement of Lymphangiogenesis In Vitro via the Regulations of HIF-1𝜶 Expression and Nuclear Translocation by Deoxyshikonin

Figure 3

Effect of deoxyshikonin on VEGF-C, -A, and -D mRNA levels and regulation of HIF-1α during cord formation of HMVEC-dLy on Matrigel. Cells were exposed with or without 0.8 μM deoxyshikonin for 6 h and then underwent experiments. (a) The relative length of cords was measured using an Angiogenesis Image Analyzer. (b), (c) VEGF-C, -A, and -D and also HIF-1α mRNA levels were detected by real-time PCR. (d) The HIF-1α protein level was determined by immunoprecipitation and Western blotting. The results were analyzed by scanning and Scion Image software. (e) HIF-1α nuclear translocation as determined by immunofluorescence microscopy. Similar results were obtained in three independent experiments; ^∗𝑃<0.05, ^∗∗𝑃<0.01 compared with their control.

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