Enhancement of Lymphangiogenesis In Vitro via the Regulations of HIF-1 Expression and Nuclear Translocation by Deoxyshikonin
Figure 4
Deoxyshikonin regulates VEGF-C mRNA levels and cord formation of HMVEC-dLy via HIF-1α. After transfection with control siRNA or siRNA for HIF-1α, HMVEC-dLy cells were seeded on Matrigel and incubated with or without 0.8 μM deoxyshikonin for 2–6 h, and then cells were subjected to real-time PCR and cord formation assay. (a) HIF-1α mRNA levels. (b) VEGF-C mRNA levels. (c) Relative length of cord formations. Similar results were obtained in three independent experiments; , compared with the control.