PXR-Mediated Upregulation of CYP3A Expression by Herb Compound Praeruptorin C from Peucedanum praeruptorum Dunn

Figure 2

Effects of PC on Cyp3a11 mRNA expression in PXR knockdown mice primary hepatocytes cells. (a) Mice primary hepatocyte were transfected with negative control siRNA (50 nM) or PXR siRNAs (50 nM). PXR mRNA levels were analyzed by real-time PCR. ^∗∗𝑃<0.01. (b) Mice primary hepatocyte were transfected with negative control (50 nM) or PXR siRNAs (50 nM) then were treated with 10  μM DEX and 2.5, 10, and 40  μM PC for 72 h, respectively. The Cyp3a11 mRNA expression was analyzed using real-time quantitative PCR.^∗𝑃<0.05, ^∗∗𝑃<0.01, for compared with the blank in control siRNA transfected groups, ^#𝑃<0.05, ^##𝑃<0.01 for comparison between negative control siRNA and PXR siRNA. Values are expressed as mean ± S.E.M (𝑛=3).

(a)

(b)