Research Article

PXR-Mediated Upregulation of CYP3A Expression by Herb Compound Praeruptorin C from Peucedanum praeruptorum Dunn

Figure 5

Determination of CYP3A4 catalytic activity. LS174T cells were transfected or untransfected with pSG5-hPXR expression plasmids for 6 h. The LS174T cells were treated with vehicle controls (0.1% DMSO); 10 μM RIF; and 2.5, 10, and 40 μM PC for 72 h, respectively. Total protein was isolated; total protein (1 mg/mL) was incubated with NIF and NADPH. The concentration of the DNIF, which is the metabolite of NIF through CYP3A4 pathway, was determined using the previously developed LC-MS/MS method. , , compared to control (DMSO) treatment group with PXR expression plasmid. Values are expressed as mean ± S.E.M ( ).
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