Intestinal Protective Effects of Herbal-Based Formulations in Rats against Neomycin Insult

Table 2

The sequences of the primers employed in qRT-PCR analysis of rat stool bacterial DNA targeting 16S rRNA gene of the universal bacteria or Lactobacillus spp.

Target gene	PS	Sequence (5′–3′)	OAT	References
Universal bacteria	F	CCTACGGGAGGCAGCAG	60°C	[17]
	R	ATTACCGCGGTGCTGG
Lactobacillus spp.	F	GAGGCAGCAGTAGGGAATCTTC	60°C	[18]
	R	GGCCAGTTACTACCTCTATCCTTCTTC

PS: primer sets; F: forward; R: reverse; OAT: optimized annealing temperature.