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Evidence-Based Complementary and Alternative Medicine
Volume 2013, Article ID 161976, 11 pages
Research Article

Potential Osteoporosis Recovery by Deep Sea Water through Bone Regeneration in SAMP8 Mice

1Stem Cell Research Center, Taipei Medical University, Taipei, Taiwan
2Graduate Institute of Biomedical Materials and Tissue Engineering, Taipei Medical University, Taipei, Taiwan
3School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan
4Department of Urology, Taipei Medical University Hospital, Taipei, Taiwan
5Department of Food and Nutrition, Providence University, Taichung, Taiwan
6Graduate Institute of Oral Rehabilitation Sciences, Taipei Medical University, Taipei, Taiwan
7Department of Family Medicine, Taipei Medical University, Wan Fang Hospital, Taipei, Taiwan
8Department of Orthopedic Surgery, The Affiliated Hospital, Guangdong Medical College, Zhanjiang 524001, China
9Translational Research Laboratory, Cancer Center, Taipei Medical University, Taipei, Taiwan
10Cancer Center, Taipei Medical University Hospital, Taipei, Taiwan

Received 3 January 2013; Revised 22 June 2013; Accepted 27 June 2013

Academic Editor: Wei-Chiang Lin

Copyright © 2013 Hen-Yu Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The aim of this study is to examine the therapeutic potential of deep sea water (DSW) on osteoporosis. Previously, we have established the ovariectomized senescence-accelerated mice (OVX-SAMP8) and demonstrated strong recovery of osteoporosis by stem cell and platelet-rich plasma (PRP). Deep sea water at hardness (HD) 1000 showed significant increase in proliferation of osteoblastic cell (MC3T3) by MTT assay. For in vivo animal study, bone mineral density (BMD) was strongly enhanced followed by the significantly increased trabecular numbers through micro-CT examination after a 4-month deep sea water treatment, and biochemistry analysis showed that serum alkaline phosphatase (ALP) activity was decreased. For stage-specific osteogenesis, bone marrow-derived stromal cells (BMSCs) were harvested and examined. Deep sea water-treated BMSCs showed stronger osteogenic differentiation such as BMP2, RUNX2, OPN, and OCN, and enhanced colony forming abilities, compared to the control group. Interestingly, most untreated OVX-SAMP8 mice died around 10 months; however, approximately 57% of DSW-treated groups lived up to 16.6 months, a life expectancy similar to the previously reported life expectancy for SAMR1 24 months. The results demonstrated the regenerative potentials of deep sea water on osteogenesis, showing that deep sea water could potentially be applied in osteoporosis therapy as a complementary and alternative medicine (CAM).