Triptolide-Mediated Apoptosis by Suppression of Focal Adhesion Kinase through Extrinsic and Intrinsic Pathways in Human Melanoma Cells
Figure 2
Effect of TPL on apoptosis of SK-MEL-2 cells. SK-MEL-2 cells were treated with 0, 30, 50, and 70 nM of TPL for 24 h. (a) Cells morphology was evaluated under the microscope. (b) Cells were washed, fixed, stained with propidium iodide (PI), and analyzed for DNA content by flow cytometry. (c) Apoptotic cells were measured by Annexin V assay and followed by analysis with flow cytometry.
