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Evidence-Based Complementary and Alternative Medicine
Volume 2013, Article ID 190281, 8 pages
Research Article

Suppressions of Migration and Invasion by Cantharidin in TSGH-8301 Human Bladder Carcinoma Cells through the Inhibitions of Matrix Metalloproteinase-2/-9 Signaling

1Department of Physiology, China Medical University, Taichung 404, Taiwan
2Department of Chinese Medicine, E-DA Hospital/I-Shou University, Kaohsiung 824, Taiwan
3Department of Life Sciences, National Chung Hsing University, Taichung 402, Taiwan
4Department of Pharmacology, China Medical University, Taichung 404, Taiwan
5Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, St. Martin De Porres Hospital, Chiayi 600, Taiwan
6School of Chinese Medicine, China Medical University, Taichung 404, Taiwan
7Special Class of Healthcare, Industry Management, Central Taiwan University of Science and Technology, Taichung 406, Taiwan
8Department of Urology, Jen-Ai Hospital, Taichung 412, Taiwan
9School of Pharmacy, China Medical University, Taichung 404, Taiwan
10Department of Biological Science and Technology, China Medical University, Taichung 404, Taiwan
11Department of Microbiology, China Medical University, Taichung 404, Taiwan
12Department of Biotechnology, Asia University, Taichung 413, Taiwan

Received 11 October 2012; Revised 10 December 2012; Accepted 11 December 2012

Academic Editor: Wei Jia

Copyright © 2013 Yi-Ping Huang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Cancer metastasis becomes an initial cause of cancer death in human population. In many cancers, it has been shown that the high levels of matrix metalloproteinase (MMP)-2 and/or MMP-9 are associated with the invasive phenotypes of cancer cells. In this study, we investigated the effects of cantharidin, a derivative of blister beetles which is one of the traditional Chinese medicines, on the adhesion, migration, and invasion of human bladder cancer TSGH-8301 cells. Cantharidin effectively suppressed TSGH-8301 cell adhesion, migration, and invasion in a concentration-dependent manner. Results from Western blotting, RT-PCR, and gelatin zymography assays indicated that cantharidin blocked the protein levels, gene expression (mRNA), and activities of MMP-2 and -9 in TSGH-8301 cells. Cantharidin also significantly suppressed the protein expressions of p-p38 and p-JNK1/2 in TSGH-8301 cells. Taken together, cantharidin was suggested to present antimetastatic potential via suppressing the levels of MMP-2 and MMP-9 expression that might be mediated by targeting the p38 and JNK1/2 MAPKs pathway in TSGH-8301 human bladder cancer cells.