Serum Containing Tao-Hong-Si-Wu Decoction Induces Human Endothelial Cell VEGF Production via PI3K/Akt-eNOS Signaling

Figure 6

Effects of different concentrations of TSD medicated serum on expression of p-Akt, total Akt and p-eNOS. Cells were treated with blank serum (5–10%) or TSD medicated serum (5–10%) for 24 h. Cells were lysed, and the expression was determined by western blot with specific primary antibody to p-Akt, total Akt, and p-eNOS; bands were displayed with secondary antibody and ECL system. Data were expressed as mean ± SD, 𝑛=3. ^∗𝑃<0.05 and ^∗∗𝑃<0.01 compared with p-eNOS of 5% blank serum group, ^Δ𝑃<0.05 and ^ΔΔ𝑃<0.01 compare with p-Akt of 5% blank serum group.