Moxibustion Inhibits the ERK Signaling Pathway and Intestinal Fibrosis in Rats with Crohn’s Disease

Table 1

The sequence of the forward primer and reverse primer used for fluorescent quantitative PCR assays.

Name	Primer sense	Sequence (5′→3′)	Amplification product (bp)
GAPDH	Forward	5′-CCGAGGGCCCACTAAAGG-3′	116 bp
	Reverse	5′-GCTGTTGAAGTCACAGGAGACAA-3′
Ras	Forward	5′-TGGTGGGCAACAAGTGTGAC-3′	181 bp
	Reverse	5′-GGGTTCAGTTTCCGCAGTTTATG-3′
Raf-1	Forward	5′-CAGGAGCACAAAGGTAAGAAAGC-3′	201 bp
	Reverse	5′-GCCACAAGTCTGACATCGAAATC-3′
MEK-1	Forward	5′-AATATGTCAGGTTCAAGGAAAGGAC-3′	169 bp
	Reverse	5′-AGGTAAGGACGAAGCCACAAG-3′
ERK-1/2	Forward	5′-TATATACATTCAGCTAATGTTCTGC-3′	169 bp
	Reverse	5′-TCTACCTGCAGCTGGAACCTCTC-3′