The Effects of Brazilian Green Propolis against Excessive Light-Induced Cell Damage in Retina and Fibroblast Cells

Figure 4

Effects of constituents of propolis on cell damage induced by UVA irradiation in an NB1-RGB culture. ((a)–(d)) Cell viability was assessed by immersing cells in WST-8 solution for 6 h at 37°C, with absorbance recorded at 492 nm. UVA induced a decrease in cell viability. (a) 3,5-Di-O-caffeoylquinic acid, (b) 3,4-di-O-caffeoylquinic acid, (c) chlorogenic acid, and (d) p-coumaric acid at 1 and 3 μg/mL significantly inhibited cell damage, respectively (versus cells treated with UVA irradiation alone). Data are shown as means ± S.E.M. (𝑛=6). **𝑃<0.01 versus UVA exposure plus the vehicle-treated group and ^##𝑃<0.01 versus control. CGA: chlorogenic acid, p-CA: p-coumaric acid, 3,5-CQA: 3,5-di-O-caffeoylquinic acid, and 3,4-CQA: 3,4-di-O-caffeoylquinic acid.

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