Bufalin Reverses HGF-Induced Resistance to EGFR-TKIs in EGFR Mutant Lung Cancer Cells via Blockage of Met/PI3k/Akt Pathway and Induction of Apoptosis
Figure 4
Bufalin combined with EGFR-TKIs overcomes resistance to EGFR-TKIs induced by HGF via inhibition of Met/PI3k/Akt pathway. (a) HCC827 cells were treated with bufalin (20 nM), SU11274 (5 μM), and/or gefitinib (1 μM) for 24 h, in the presence or absence of HGF (30 ng). (b) H1975 cells were treated with bufalin (20 nM), SU11274 (5 μM), and/or BIBW2992 (0.1 μM) for 24 h, in the presence or absence of HGF (30 ng). Then cells were lyzed, and the associated proteins were detected by western blotting.
