Evidence-Based Complementary and Alternative Medicine / 2013 / Article / Fig 4

Research Article

Blockade of Airway Inflammation by Kaempferol via Disturbing Tyk-STAT Signaling in Airway Epithelial Cells and in Asthmatic Mice

Figure 4

Temporal activation of Tyk2 (a) and its blockade by kaempferol in LPS-exposed epithelial cells. BEAS-2B cells were cultured with 2 μg/mL LPS (b) or 10 ng/mL IL-8 (c) in the absence and presence of 1–20 μM kaempferol for 8 h. Total cell protein extracts were subjected to western blot analysis with a primary antibody against phosphorylated Tyk2. β-actin was used as an internal control. The bar graphs (mean SEM, ) in the bottom panels represent quantitative densitometric results. Means without a common letter differ, .
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(a)
250725.fig.004b
(b)
250725.fig.004c
(c)

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