Evidence-Based Complementary and Alternative Medicine / 2013 / Article / Tab 1

Research Article

Electroacupuncture-Induced Dynamic Processes of Gene Expression Levels of Endogenous Opioid Peptide Precursors and Opioid Receptors in the CNS of Goats

Table 1

The quantitative real-time PCR primers of different genes.

Gene Upstream primerDownstream primerLength of PCR product Referenced GenBank no.

-Actin5′-ACACGGTGCCCATCTACGA-3′5′-CCTTGATGTCACGGACGATTT-3′159 bpAF481159
PENK5′-GGCGACCGTGAGGGCAGAATG-3′5′-GCAGGTTTCCCAGGTCTTGAG-3′139 bpNM174141
POMC5′-GGCGGCCGAGAAGAAGGAC-3′5′-CTTGATGATGGCGTTTTTGA-3′148 bpNM001009266
PDYN5′-CTTTCCTCACTCCCTTCACCG-3′5′-TCTCCACCAGGCTGCTACTCA-3′186 bpNM174139
-receptor5′-TGCTCTCCATTGACTACTACA-3′5′-AAGAGGAACACGGCAGATTTTG-3′289 bpNM001191148
-receptor5′-AGTGTGTTATGGGCTGATGA-3′5′-GCGTCCAGCAGACAATGAACACAG-3′137 bpAF266480
-receptor5′-CGTCTTCGTCGTCTGCTGG-3′5′-CAAGGAAGGCGTAGAGAATGG-3′158 bpDQ065757