Tetrandrine Suppresses Cancer Angiogenesis and Metastasis in 4T1 Tumor Bearing Mice
Figure 1
Antiproliferative activity of TET in HUVECs. (a) MTT assay. Subconfluent HUVECs were treated with indicated concentrations of TET for 48 h. The cells were then subjected to MTT assay. Each assay condition was done in triplicate (). (b) Crystal violet assay. HUVECs were treated with TET at the indicated concentrations for 24 h. The cells were subjected to crystal violet assay as described in Section 2. (c) Cell cycle analysis. Cell cycle distribution of HUVECs was analyzed by flowcytometry. Cells were treated with 1 μM TET for 24 h and fixed, and then nuclear DNA was labeled with PI. Histogram display DNA content (-axis [PE-A]: PI-fluorescence) versus cell counts (-axis). (d) ERK, p-ERK, and NFκB expressions in HUVECs. The expression of ERK, p-ERK, and NFκB was detected by western blot.
