Figure 3: (a)-(b) Effect of β-elemene on the cell viability of CD44+ cells. CD44+ and CD44− cells were treated with 50, 100, 150, or 200 μg/mL β-elemene for 24, 48, or 72 h. The cell viability was determined using an MTT assay. Dots: mean of six independent experiments; bars: SD. (c)-(d). β-Elemene inhibited Notch-1 and Hes1 expressions in vitro. CD44+ and CD44− cells were seeded in 96-well plates at a density of 5 × 104 cells/well and treated with a series of β-elemene doses over 48 h. Cellular lysates were subjected to western blot analysis with antibodies against Notch-1, Hes1, and β-actin (loading control). The densitometry analysis results of the Notch-1 and Hes1 bands were normalized to β-actin using NIH Image J from six independent experiments. The data are expressed as arbitrary units.