Bioactive Lipidic Extracts from Octopus (Paraoctopus limaculatus): Antimutagenicity and Antiproliferative Studies

Table 2

Anti-mutagenic potential of AFB₁^a-spiked fractions obtained from thin layer chromatography fractionation of fraction RB (𝑅_𝑓 = 0.18–0.78) from TLC 1 (average^b TA100 and TA98 revertants/plate, with S9).

Extract fraction^c	Fraction dilution^c
	1×10^0	1×10^−1	1×10^−2	1×10^−3
TA98
RB1 (𝑅𝑓 = 0.18–0.78)	464±98	1230±64	933±59	1023±53
RB2 (𝑅𝑓 = 0.78–1.00)	82±13	548±48	632±56	955±65
TA100
RB1 (𝑅𝑓 = 0.18–0.78)	564±37	1355±94	1299±105	1283±88
RB2 (𝑅𝑓 = 0.78–1.00)	315±20	348±25	668±32	740±13

^aAFB₁ (used as positive control) tested at 500 and 1000 ng/plate induced 1073±29 and 1841±102 and 1229±37 and 2286±103 revertants/plate for TA98 and TA100, respectively. Spontaneous revertants were 52±4 and 160±4 for TA98 and TA100, respectively.
^bValues are means of three replicates ± SEM.
^cExtracts were diluted and spiked with enough toxin to yield 500 ng of pure AFB₁/plate.