Bioactive Lipidic Extracts from Octopus (Paraoctopus limaculatus): Antimutagenicity and Antiproliferative Studies

Table 5

Anti-mutagenic potential of AFB₁^a-spiked fractions obtained from thin layer chromatography fractionation of fraction RB213 (𝑅_𝑓 = 0.68–1.00) from TLC 2 (average^b TA100 and TA98 revertants/plate, with S9).

Extract fraction^c	Fraction dilution^c
	1×10^0	1×10^−1	1×10^−2	1×10^−3
TA98
RB2132 (𝑅𝑓 = 0.85–0.94)	79±11	245±25	409±29	502±28
RB2133 (𝑅𝑓 = 0.94–1.00)	399±35	435±49	489±41	576±21
TA100
RB2132 (𝑅𝑓 = 0.85–0.94)	178±13	389±41	603±59	918±28
RB2133 (𝑅𝑓 = 0.94–1.00)	499±35	829±63	1237±128	1465±121

^aAFB₁ (used as positive control) tested at 500 ng/plate induced 491 ± 33 and 1283 ± 78 revertants/plate for TA98 and TA100, respectively. Spontaneous revertants were 54±3 and 128±11 for TA98 and TA100, respectively.
^bValues are means of three replicates ± SEM.
^cExtracts were diluted and spiked with enough toxin to yield 500 ng of pure AFB₁/plate.