Bioactive Lipidic Extracts from Octopus (Paraoctopus limaculatus): Antimutagenicity and Antiproliferative Studies

Table 6

Anti-mutagenic potential of AFB₁^a-spiked fractions obtained from thin layer chromatography fractionation of fraction RB2132 (𝑅_𝑓 = 0.68–1.00) from TLC 2 (average^b TA100 and TA98 revertants/plate, with S9).

Extract fraction^c	Fraction dilution^c
	1×10^0	1×10^−1	1×10^−2	1×10^−3
TA98
RB21321 (𝑅𝑓 = 0.84–0.90)	169±11	263±17	461±29	534±48
RB21322 (𝑅𝑓 = 0.90–1.00)	150±14	301±29	423±38	401±45
TA100
RB21321 (𝑅𝑓 = 0.84–0.90)	278±23	492±41	603±59	918±28
RB21322 (𝑅𝑓 = 0.90–1.00)	423±39	734±52	847±78	907±37

^aAFB₁ (used as positive control) tested at 500 ng/plate induced 536±39 and 958±88 revertants/plate for TA98 and TA100, respectively. Spontaneous revertants were 20±4 and 120±9 for TA98 and TA100, respectively.
^bValues are means of three replicates ± SEM.
^cExtracts were diluted and spiked with enough toxin to yield 500 ng of pure AFB₁/plate.