Primo Vascular System in the Subarachnoid Space of a Mouse Brain

Figure 3

(a) Stereomicroscopic image of the dura mater taken from the mouse brain. The PN is indicated with a circle that is 3,900 μm from the λ point (arrow). The specimen is sample no. 10 in Table 1. PV: primo vessel, PN: primo node, SSS: superior sagittal sinus, and TS (R, L): transverse sinus right, left, respectively. (b) Phase-contrast microscopic image of the PN. It has an eggplant shape, and its size is 180 μm × 420 μm. (c) Fluorescent image of the nuclei distribution of the same PN after DAPI staining. The DAPI staining was not clearly seen with the phase-contrast microscope because the PN was too thick. (d) The fluorescence microscope with tissues MVX-10 showed a better image of the DAPI staining. (e) The f-actins of cells in the same PN are shown with phalloidin staining. (f) A 3D image of the PN stained with DAPI and phalloidin taken with a confocal laser scanning microscope. (g) The primo vessel (PV) attached to the PN is shown. Primo vessel (dotted arrow).