Effect of Berberine on PPARα/NO Activation in High Glucose- and Insulin-Induced Cardiomyocyte Hypertrophy

Table 1

Oligonucleotide sequences for real-time RT-PCR.

Gene	Forward (5′-3′ orientation)	Reverse (5′-3′ orientation)
ANF	TGACAGGATTGGAGCCCAGAG	TCGAGCAGATTTGCTGTTATCTTC
eNOS	TGCAACAAACCGAGGCAATC	CACCAGCTGGCTGTTCCAGA
PPARα	CTGACATTTGTGACTGGTCAAGCTC	TTTCCAGGTCATCTGCTTCAAGTG
β-actin	GGCCAACCGTGAAAAGATGA	CAGCCTGGATGGCTACGTACA

ANF: atrial natriuretic factor; eNOS: endothelial nitric oxide synthase; PPARα: peroxisome proliferator-activated receptor-α.