Ellagic Acid Reduces Adipogenesis through Inhibition of Differentiation-Prevention of the Induction of Rb Phosphorylation in 3T3-L1 Adipocytes
Figure 6
EA inhibits differentiation-induced C/EBPα DNA-binding activity and its following protein expression. Nuclear fractions were prepared at different time after induction of differentiation and assessed for DNA-binding activity of C/EBPα, β. Results are the mean ± SD of 3 experiments (a and b). Solubilised protein from duplicate cultures was immunoblotted with antibodies against C/EBPα or β-actin (loading control). Immunoblots shown are representative of 3 independent experiments (c). Densitometric data are expressed as mean ± SD of 3 independent experiments, each performed in duplicate (d). compared to control (without EA treatment).