Carvacrol Protects against Hepatic Steatosis in Mice Fed a High-Fat Diet by Enhancing SIRT1-AMPK Signaling
Figure 5
Reduced hepatic TLR2- and 4-mediated signaling and plasma levels of inflammatory markers in CSD-fed mice. (a) Hepatic mRNA expression levels of TLR2, TLR4 and related genes normalized to GAPDH expression relative to ND-fed mice. (b) Representative blots of hepatic IRF3 and phosphorylated IRF3 proteins in total liver extracts from the ND-, HFD-, and CSD-fed mice. Blots were quantified and the data are presented as the ratio of phosphorylated IRF3 to native protein, with values normalized to ND-fed mice. (c and d) Plasma MCP1 and TNFα levels. Values are means ± SEM from 8 animals, *.
