|
| Gene description | Primers | Sequences (5′ → 3′) | Annealing
temperature
(°C) | PCR
product (bp) |
|
| Sirtuin1 (SIRT1) | F | CAGAACCACCAAAGCGGAAA |
55 |
693 |
| R | GGCACTTCATGGGGTATAGA |
Liver X receptor
(LXRα) | F | TCCTACACGAGGATCAAGCG |
55 |
119 |
| R | AGTCGCAATGCAAACACCTG |
| SREBP1c (SREBP1c) | F | TTGTGGAGCTCAAAGACCTG |
55 |
94 |
| R | TGCAAGAAGCGGATGTAGTC |
| CD36 antigen (CD36) | F | ATGACGTGGCAAAGAACAGC |
55 |
160 |
| R | GAAGGCTCAAAGATGGCTCC |
| Lipoprotein lipase (leptin) | F | CTCCAAGGTTGTCCAGGGTT |
55 |
143 |
| R | AAAACTCCCCACAGAATGGG |
| Fatty acid synthase (FAS) | F | AGGGGTCGACCTGGTCCTCA |
65 |
132 |
| R | GCCATGCCCAGAGGGTGGTT |
| Carnitine palmitoyltransferase I (CPT1) | F | CTCTGCTGGCCGTTGTTGT |
55 |
120 |
| R | GGCAAGTTCTGCCTCACGTA |
| Sterol regulatory element-binding protein-2 (SREBP2) | F | CACAATATCATTGAAAAGCG |
60 |
200 |
| R | TTTTTCTGATTGGCCAGCTT |
| 3-Hydroxy-3-methylglutaryl-CoA reductase (HMGCR) | F | TAAGATTCAACAACTCTGCT |
55 |
101 |
| R | TGTGGCCAGGAGTTTGGTGA |
| Farnesyl diphosphate synthase (FDPS) | F | ATGGAGATGGGCGAGTTCTT |
60 |
80 |
| R | CCGACCTTTCCCGTCACA |
| Cytochrome P450, family 51 (CYP51) | F | ACGCTGCCTGGCTATTGC |
55 |
76 |
| R | TTGATCTCTCGATGGGCTCTATC |
| Low-density lipoprotein receptor (LDLR) | F | AGGCTGTGGGCTCCATAGG |
60 |
72 |
| R | TGCGGTCCAGGGTCATCT |
| ATP-binding cassette, sub-family G, member 5 (ABCG5) | F | CGTGGCGGACCAAATGA |
55 |
155 |
| R | CGCTCGCCACTGGAAATT |
| ATP-binding cassette, sub-family G, member 8 (ABCG8) | F | TGCCCACCTTCCACATGTC |
60 |
60 |
| R | ATGAAGCCGGCAGTAAGGTAGA |
| Cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1) | F | CAGGGAGATGCTCTGTGTTCA |
60 |
121 |
| R | AGGCATACATCCCTTCCGTGA |
| Cytochrome P450, family 8, subfamily A, polypeptide 1 (CYP8B1) | F | AAGGCTGGCTTCCTGAGCTT |
60 |
74 |
| R | AACAGCTCATCGGCCTCATC |
| Acyl-coenzyme A: cholesterol acyltransferase (ACAT1) | F | AGCGAGACAGATGCTCATGC |
55 |
107 |
| R | CAACCAAACCTCCGTCACTG |
| Toll-like receptor 2 (TLR2) | F | GAGCATCCGAATTGCATCAC |
55 |
120 |
| R | TATGGCCACCAAGATCCAGA |
| Toll-like receptor 4 (TLR4) | F | TCGAATCCTGAGCAAACAGC |
55 |
199 |
| R | CCCGGTAAGGTCCATGCTAT |
| Toll-interleukin 1 receptor domain-containing adaptor protein (Tirap) | F | GCTTCCAGGGGATCTGATGT |
55 |
183 |
| R | AAGCAAGCCTACCACGGACT |
| TIR-domain-containing adapter-inducing interferon-β (TRIF) | F | ATGGATAACCCAGGGCCTT |
55 |
528 |
| R | TTCTGGTCACTGCAGGGGAT |
| Interferon regulatory factor 5 (IRF5) | F | ACCCGGATCTCAAAGACCAC |
55 |
166 |
| R | TTATTGCATGCCAACTGGGT |
| TNF alpha (TNFα) | F | TGTCTCAGCCTCTTCTCATT |
55 |
156 |
| R | AGATGATCTGAGTGTGAGGG |
| Interleukin 1 beta (IL-1β) | F | GTTGACGGACCCCAAAAGAT |
55 |
129 |
| R | TGATACTGCCTGCCTGAAGC |
| Interferon beta (IFNβ) | F | TGGAGCAGCTGAATGGAAAG |
55 |
122 |
| R | GAGCATCTCTTGGATGGCAA |
| Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | F | CCCATGTTTGTGATGGGTGT |
55 |
161 |
| R | GTGATGGCATGGACTGTGGT |
|
