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Evidence-Based Complementary and Alternative Medicine
Volume 2013, Article ID 290675, 8 pages
http://dx.doi.org/10.1155/2013/290675
Research Article

Propolis Induces Chondroitin/Dermatan Sulphate and Hyaluronic Acid Accumulation in the Skin of Burned Wound

1Department of Community Pharmacy, Medical University of Silesia, ul. Kasztanowa 3, 41-200 Sosnowiec, Poland
2Department of Clinical Chemistry and Laboratory Diagnostics, Medical University of Silesia, ul. Kasztanowa 3, 41-200 Sosnowiec, Poland
3Center of Experimental Medicine, Medical University of Silesia, ul. Kasztanowa 3, 41-200 Sosnowiec, Poland
4Department of Statistics, Medical University of Silesia, ul. Kasztanowa 3, 41-200 Sosnowiec, Poland

Received 7 January 2013; Accepted 9 February 2013

Academic Editor: Ewelina Szliszka

Copyright © 2013 Pawel Olczyk et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Changes in extracellular matrix glycosaminoglycans during the wound repair allowed us to apply the burn model in which therapeutic efficacy of propolis and silver sulfadiazine was compared. Burns were inflicted on four pigs. Glycosaminoglycans isolated from healthy and burned skin were quantified using a hexuronic acid assay, electrophoretic fractionation, and densitometric analyses. Using the reverse-phase HPLC the profile of sulfated disaccharides released by chondroitinase ABC from chondroitin/dermatan sulfates was estimated. Chondroitin/dermatan sulfates and hyaluronic acid were found in all samples. Propolis stimulated significant changes in the content of particular glycosaminoglycan types during burn healing. Glycosaminoglycans alterations after silver sulfadiazine application were less expressed. Propolis maintained high contribution of 4-O-sulfated disaccharides to chondroitin/dermatan sulfates structure and low level of 6-O-sulfated ones throughout the observed period of healing. Propolis led to preservation of significant contribution of disulfated disaccharides especially 2,4-O-disulfated ones to chondroitin sulfates/dermatan sulfates structure throughout the observed period of healing. Our findings demonstrate that propolis accelerates the burned tissue repair by stimulation of the wound bed glycosaminoglycan accumulation needed for granulation, tissue growth, and wound closure. Moreover, propolis accelerates chondroitin/dermatan sulfates structure modification responsible for binding growth factors playing the crucial role in the tissue repair.