Evidence-Based Complementary and Alternative Medicine / 2013 / Article / Fig 2

Research Article

Antcin C from Antrodia cinnamomea Protects Liver Cells Against Free Radical-Induced Oxidative Stress and Apoptosis In Vitro and In Vivo through Nrf2-Dependent Mechanism

Figure 2

Antcin C induced antioxidant genes expression in AAPH-induced HepG2 cells. (a) HepG2 cells were preincubated with antcin C (5–20 μM) or silymarin (100 μM) for 2 h, and then exposed to AAPH (10 mM) for 24 h. Total cell lysates were prepared and subjected to western blot analysis to monitor the expression levels of antioxidant proteins, including HO-1, NQO-1, -GCLC, and SOD. -actin served as an internal control. (b) The effect of antcin C on mRNA levels of antioxidant genes. HepG2 cells were pre-incubated with antcin C (5–20 μM) or silymarin (100 μM) for 2 h, and then exposed to AAPH (10 mM) for 6 h. The mRNA levels of HO-1, NQO-1, -GCLC were semiquantified by RT-PCR analyses. -Actin was used as a loading control. (c) HepG2 cells were pre-incubated with antcin C (20 μM) or silymarin (100 μM) for 2 h, and then exposed to AAPH (10 mM) for 6 h. Q-PCR analysis was performed to monitor the expression levels of HO-1, NQO-1, -GCLC, and Nrf2 mRNA levels. Values represent the mean ± SD of three independent experiments. , , and were considered significant for sample versus AAPH. was considered significant for control versus AAPH.
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