Evidence-Based Complementary and Alternative Medicine / 2013 / Article / Fig 4

Research Article

Antcin C from Antrodia cinnamomea Protects Liver Cells Against Free Radical-Induced Oxidative Stress and Apoptosis In Vitro and In Vivo through Nrf2-Dependent Mechanism

Figure 4

Effects of antcin C on AAPH-induced MAPK activation in HepG2 cells. ((a)–(d)) Cells were pretreated with antcin C (5–20 μM) or silymarin (100 μM) or MAPK inhibitors (30 μM) for 2 h, then exposed to AAPH (10 mM) for 15 min-1 h. Total cell lysates or the nuclear fraction were prepared and subjected to western blot analysis. Phosphorylated and nonphosphorylated forms of p38 MAPK, JNK1/2 and ERK1/2, PKC, and PI3K proteins were examined. -actin was used as a loading control.