The Inhibitory Effect of Ellagic Acid on Cell Growth of Ovarian Carcinoma Cells
Anchorage-independent growth and anoikis in EA-treated ovarian carcinoma cells. Cells were cultured in methylcellulose-coated 6-well plates and treated with different concentrations of EA as indicated. The formed cell clusters were photographed under a microscope (a). The suspended cell clusters were scattered and stained with trypan blue. The surviving cells were counted using a haemocytometer (b). The same scattered cells were stained with annexin V-FITC and then subjected to flow cytometry for apoptosis analysis (c). Cells were suspended in soft agar and incubated with different concentrations of EA as indicated for 14 days for anchorage-independent growth in soft agar (d). The data reported are the averages of three independent experiments and are expressed as means ± SD.