Evidence-Based Complementary and Alternative Medicine / 2013 / Article / Fig 7

Research Article

Molecular Mechanisms of Large-Conductance Ca2+-Activated Potassium Channel Activation by Ginseng Gintonin

Figure 7

Double mutations of CaM kinase II and Ca2+ bowl or RCK domain further attenuate gintonin-mediated BKCa channel activation. (a) Time-current relationship after application of gintonin (10 μg/mL) or ginsenoside Rg3 (100 μM) for 60 s in oocytes coexpressing BKCa channels and CaM kinase II + Ca2+ bowl mutants. Insets, the representative peak outward current amplitude at +40 mV from a holding potential of −80 mV was measured in the presence of gintonin or ginsenoside Rg3. (b) Time-current relationship after application of gintonin (10 μg/mL) or ginsenoside Rg3 (100 μM) for 60 s in oocytes coexpressing BKCa channels and CaM kinase II + RCK domain mutants. Inset, the representative peak outward current amplitude at +40 mV from a holding potential of −80 mV was measured in the presence of gintonin or ginsenoside Rg3. (c) Coexpression of BKCa channels with CaM kinase II + Ca2+ bowl mutants caused a further rightward shift of the gintonin concentration-response curve. (d) Coexpression of BKCa channels with CaM kinase II + RCK domain mutants caused a further rightward shift of the gintonin concentration-response curve (mean ± S.E.M; oocytes each).
323709.fig.007a
(a) T462/D994A
323709.fig.007b
(b) T462/D433A
323709.fig.007c
(c)
323709.fig.007d
(d)

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