Prenylated Flavonoids from Morus alba L. Cause Inhibition of G1/S Transition in THP-1 Human Leukemia Cells and Prevent the Lipopolysaccharide-Induced Inflammatory Response
Figure 4
(a) Effects of M. alba L. prenylated flavonoids and the reference drug prednisone on LPS-induced TNF-α secretion at macrophages derived from THP-1 cell line. Cells were pretreated with given compounds (10 μM), prednisone (1 μM), or the vehicle (DMSO) only. After 1 h of incubation, the inflammatory response was induced by LPS (except for the control cells). Results are expressed as means ± S.E. for three independent experiments. *Significant difference in comparison to vehicle-treated cells (), ***significant difference in comparison to vehicle-treated cells (), #significant difference in comparison to prednisone-treated cells (), and ##significant difference in comparison to prednisone-treated cells (). (b) Effects of M. alba L. prenylated flavonoids and the reference drug prednisone on LPS-induced IL-1β secretion at macrophages derived from THP-1 cell line. Cells were pretreated with given compounds (10 μM), prednisone (1 μM), or the vehicle (DMSO) only. After 1 h of incubation, the inflammatory response was induced by LPS (except for the control cells). Results are expressed as means ± S.E. for three independent experiments. **Significant difference in comparison to vehicle-treated cells (); ##significant difference in comparison to prednisone-treated cells (). (c) Effects of M. alba L. prenylated flavonoids and the reference drug prednisone on LPS-induced IL-1RA secretion at macrophages derived from THP-1 cell line. Cells were pretreated with given compounds (10 μM), prednisone (1 μM), or the vehicle (DMSO) only. After 1 h of incubation, the inflammatory response was induced by LPS (except for the control cells). Results are expressed as means ± S.E. for three independent experiments. ***Significant difference in comparison to vehicle-treated cells (). (d) Ratio IL-1β/IL-1RA production calculated for macrophages derived from THP-1 cell line. Values were obtained from ELISA measurements of individual cytokines as it is described in Figures 2 and 3. Results are expressed as means ± S.E. for three independent experiments. A.U. = arbitrary unit. ***Significant difference in comparison to vehicle-treated cells (); ###significant difference in comparison to prednisone-treated cells ().