Research Article

Gross Morphological Features of the Organ Surface Primo-Vascular System Revealed by Hemacolor Staining

Figure 3

Confocal laser scanning microscopic images of whole PVS tissue and cells stained by acridine orange. (a) Unsectioned longitudinal image of a whole PVS composed of two PNs connected by a PV. Note that the tissue is densely filled with cells stained with green (majority) or dark brown. Some cells are linearly aligned along the longitudinal axis of the PV (bottom inset). (a1) PN cells with random distribution in the inner region (marked as “a1” in (a)). (a2 and a3) PN and PV cells at a high magnification (400x) (marked as “a2” and “a3” in (a)). Note the large cells (arrows) with granules stained red and small round cells (arrowheads) stained green in both the PNs and the PV. (b1, b2, and b3) Unsectioned longitudinal image of a whole PV showing an inner space according to depth of optical sectioning. Note that the inner space structure (arrows) devoid of cells is becoming darker with increasing depth of optical section.
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