Evidence-Based Complementary and Alternative Medicine / 2013 / Article / Tab 1

Research Article

Microarray Analysis of mRNA and MicroRNA Expression Profile Reveals the Role of β-Sitosterol-D-glucoside in the Proliferation of Neural Stem Cell

Table 1

Primer sequences of the reference gene and the genes selected.

GenePrimer sequenceAnnealing temperature (°C)Product length (bp)

GAPDHF: 5′-GGAAAGCTGTGGCGTGAT-3′60308
R: 5′-AAGGTGGAAGAATGGGAGTT-3′
IGF1F: 5′-CTGGCACTCTGCTTGCTCAC-3′60180
R: 5′-CTCATCCACAATGCCCGTCT-3′
cdkn1cF: 5′-CCTCCCGTTCCCTTCTTTCT-3′6096
R: 5′-CGTTCCATCGCTGTTCTGC-3′
Espl1F: 5′-TGACTACCTGGGCGTGACTG-3′6098
R: 5′-CTGGCTCTGAGATGGCACAA-3′
Pttg1F: 5′-TGGAGACAGTTGTTTGGGTGC-3′60270
R: 5′-GCTGCCTGGCTCTTCGTTAT-3′
PtpruF: 5′-ACCCTGAGCGAGAACGACA-3′60285
R: 5′-GGGATGGCTGAATAGCAAGAT-3′

To validate the mRNA expression profiling, five genes (Igf1, cdkn1c, Espl1, Pttg1, and Ptpru) were selected to performed real time-PCR. Primer sequences of the GAPDH gene and the genes selected were listed in Table 1. Igf1: Insulin-like growth factor-1; cdkn1c: cyclin-dependent kinase inhibitor 1C; Espl1: extra spindle pole bodies homolog 1, Pttg1: pituitary tumor-transforming 1; Ptpru: protein tyrosine phosphatase, receptor type, U.

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