Microarray Analysis of mRNA and MicroRNA Expression Profile Reveals the Role of β-Sitosterol-D-glucoside in the Proliferation of Neural Stem Cell

Table 1

Primer sequences of the reference gene and the genes selected.

Gene	Primer sequence	Annealing temperature (°C)	Product length (bp)
GAPDH	F: 5′-GGAAAGCTGTGGCGTGAT-3′	60	308
	R: 5′-AAGGTGGAAGAATGGGAGTT-3′
IGF1	F: 5′-CTGGCACTCTGCTTGCTCAC-3′	60	180
	R: 5′-CTCATCCACAATGCCCGTCT-3′
cdkn1c	F: 5′-CCTCCCGTTCCCTTCTTTCT-3′	60	96
	R: 5′-CGTTCCATCGCTGTTCTGC-3′
Espl1	F: 5′-TGACTACCTGGGCGTGACTG-3′	60	98
	R: 5′-CTGGCTCTGAGATGGCACAA-3′
Pttg1	F: 5′-TGGAGACAGTTGTTTGGGTGC-3′	60	270
	R: 5′-GCTGCCTGGCTCTTCGTTAT-3′
Ptpru	F: 5′-ACCCTGAGCGAGAACGACA-3′	60	285
	R: 5′-GGGATGGCTGAATAGCAAGAT-3′

To validate the mRNA expression profiling, five genes (Igf1, cdkn1c, Espl1, Pttg1, and Ptpru) were selected to performed real time-PCR. Primer sequences of the GAPDH gene and the genes selected were listed in Table 1. Igf1: Insulin-like growth factor-1; cdkn1c: cyclin-dependent kinase inhibitor 1C; Espl1: extra spindle pole bodies homolog 1, Pttg1: pituitary tumor-transforming 1; Ptpru: protein tyrosine phosphatase, receptor type, U.