Evidence-Based Complementary and Alternative Medicine / 2013 / Article / Tab 2

Research Article

Microarray Analysis of mRNA and MicroRNA Expression Profile Reveals the Role of β-Sitosterol-D-glucoside in the Proliferation of Neural Stem Cell

Table 2

RT Primer sequence of the internal control gene and the target genes for cDNA synthesis.

GenesRT primer sequence

U65′-CGCTTCACGAATTTGCGTGTCAT-3′
rno-miR-129-5p5′-GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACGCAAGCC-3′
rno-miR-322-5p5′-GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTCCAAAA-3′
rno-miR-301a-3p5′-GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACGCTTTG-3′

To validate the miRNA expression profiling, three genes were selected to performed real time-PCR. RT primer sequences of the U6 gene used as reference and the genes selected were listed in Table 2.

We are committed to sharing findings related to COVID-19 as quickly as possible. We will be providing unlimited waivers of publication charges for accepted research articles as well as case reports and case series related to COVID-19. Review articles are excluded from this waiver policy. Sign up here as a reviewer to help fast-track new submissions.