Evaluation of In Vitro Anti-Inflammatory Activities and Protective Effect of Fermented Preparations of Rhizoma Atractylodis Macrocephalae on Intestinal Barrier Function against Lipopolysaccharide Insult
Figure 9
The effect of treatment with LPS in combination with saline (LC) or with two different concentrations of fermented Rhizoma Atractylodis Macrocephalae-2 (FRAM-2) on NF-κ β activity in RAW264.7 cells. The cells were treated with the indicated concentrations of FRAM-2 for 24 h. The control cells (N) and the noncontrol cells that were assigned to treatment with LPS alone (LC) were treated with sterile saline instead of herbal extract. After this treatment, the noncontrol and control cells were treated with LPS (10 μg/mL) and PBS, respectively, for 1 h; the activation of NF-κ β was then determined. The detailed treatment regimen and experimental conditions are described in Section 2. The level of NF-κ β activity in the control cells (N) was set to 100%. The values are expressed as the mean ± SD, . The data were log-transformed prior to analysis by ANOVA. significant difference compared to control cells (); significant difference compared to cells treated with LPS plus saline (). FHE: fermented herbal extract.
