Figure 9: Comprehensive immunofluorescence images and immunoblotting analysis of the differentially expressed proteins identified by MALDI-TOF MS. (a) The differential expression and distribution of α-SNAP in H9C2 cells responded to H2O2 and quercetin (Que) were analyzed using immunofluorescence. (b) Immunoblotting was performed to validate CDK4 and STIP1 in the H9C2 cell lysate. α-Tubulin served as a loading control. Scale bar = 20 μm.