Cardioprotective Effects of Quercetin in Cardiomyocyte under Ischemia/Reperfusion Injury

Table 1

Differentially expressed proteins were listed alphabetically after 2D-DIGE and MALDI-TOF mass spectrometry analysis in H9C2 cells in response to H₂O₂ treated and pretreated with quercetin. The average ratios of these 44 spots are differentially expressed between untreated (control), H₂O₂-treated, and quercetin-pretreated followed by treatment with H₂O₂, calculated from triplicate gels. Boldface numbers represent proteins for which the changes between the H₂O₂ treatment and the control are significantly greater than changes between quercetin pretreatment followed by H₂O₂ treatment and control.

Spot no.	Swissprot no.	Protein name	Pred. MW	Pred. PI	Cov. %	MASCOT score	No. of peptides; match/; supplied	H2O2/Ctrl	Quercetin + H2O2/Ctrl	Peptide sequence	Function
1420	P63324	40S ribosomal protein S12	14858	6.82	42%	55/51	6/30	−1.25	−2.77	QAHLCVLASNCDEPMYVK,; TALIHDGLAR	Protein synthesis
691	Q9JLJ3	4-Trimethylaminobutyraldehyde dehydrogenase	54530	6.57	44%	109/56	16/66	1.68	1.9	AFEPATGR,; AGAPNGLFNVVQGGAATGQFLCQHR	Protein synthesis
473	P63039	60 kDa heat shock protein (mitochondrial)	61088	5.91	20%	61/56	8/30	1.37	1.54	AAVEEGIVLGGGCALLR,; ISSVQSIVPALEIANAHR	Chaperon
1010	P60711	Actin (cytoplasmic)	42052	5.29	23%	54/51	6/37	−1.51	−1.27	GYSFTTTAER,; SYELPDGQVITIGNER	Cytoskeleton
704	P11884	Aldehyde dehydrogenase, mitochondria	56966	6.63	41%	143/56	16/54	1.12	1.56	RVTLELGGK,; SGQQEGAK	Redox regulation
990	P54921	Alpha-soluble NSF attachment protein	33627	5.3	50%	120/56	13/39	9.85	7.69	QAEAMALLAEAER,; IEEACEIYAR	Transport
1002	P55260	Annexin A4	36168	5.31	39%	145/51	13/28	−1.92	−2.35	GDTSGDYR,; WGTDEVK	Transport/Ca^2+
396	P48037	Annexin A6	76106	5.39	52%	253/56	36/74	−1.35	−1.73	YELTGKFER,; AINEAYKEDYHK	Transport/Ca^2+
1116	P35426	Cell division protein kinase 4	34006	6.09	54%	198/56	15/32	3.5	3.22	VTLVFEHIDQDLR,; VPNGGAAGGGLPVSTVR	Cell cycle
1319	P47875	Cysteine and glycine-rich protein 1	21455	8.9	51%	58/56	6/44	−7.38	−11.9	NLDSTTVAVHGEEIYCK,; GLESTTLADKDGEIYCK	Cytoskeleton regulation
1323	P47875	Cysteine and glycine-rich protein 1	21455	8.9	54%	91/56	7/40	13.77	12.77	TVYFAEEVQCEGNSFHK,; HEEAPGHRPTTNPNASK	Cytoskeleton regulation
416	Q5XI50	E3 ubiquitin-protein ligase MARCH7	76932	7.64	16%	53/51	9/44	−2.86	−3.98	MVSGNRGTSLNDSYHSR,; CTGSLQYVHQECMK	Protein degradation
709	P62630	Elongation factor 1-alpha 1	50424	9.1	29%	87/56	11/35	−2.01	−1.1	EHALLAYTLGVK,; STTTGHLIYK	Protein synthesis
1244	O08719	Ena/VASP-like protein	42183	8.74	20%	59/51	6/43	2.27	1.63	WVPIKPGQQGFSR,; VKPAGSVNDVGLDALDLDRMK	Cytoskeleton regulation
305	Q99PF5	Far upstream element-binding protein 2	74466	6.38	41%	169/56	20/43	−2.09	−2.25	ERDQGGFGDR.; IGQQPQQPGAPPQQDYTK	Gene expression
886	P97590	Galectin-7	15333	6.43	29%	52/51	6/43	−1.41	−1.58	MPSSNVRSVEVGGDVQLHSVK,; MSATHHK	Apoptosis
1251	Q9Z1B2	Glutathione S-transferase Mu 5	27067	6.33	50%	76/56	15/54	−4.85	−9.44	ITQSNAILR,; VDIMENQIMDFR	Redox regulation
1255	P42930	Heat shock protein beta-1	22936	6.12	56%	147/56	12/65	−3.9	−4.49	KYTLPPGVDPTLVSSSLSPEGTLTVEA,; VPFSLLR	Chaperon
954	Q6RUG5	Islet cell autoantigen 1-like protein	49298	5.23	31%	54/51	9/53	−1.64	−1.6	MDSFEHLRPEDSQSVVSRMQK,; DASQELDPDTFK	Unknown
1240	O35760	Isopentenyl-diphosphate Delta-isomerase 1	26721	5.57	38%	61/56	6/58	−1.8	−1.31	MPEINASNLDEK,; AELGIPLEEVDLNEMNYLTR	Lipid synthesis
1237	Q63279	Keratin, type I cytoskeletal 19	44609	5.21	25%	51/51	10/73	4.02	3.7	QGPGPFRDYSQYFK,; MSVEADINGLRR	Cytoskeleton
1219	Q6QLM7	Kinesin heavy chain isoform 5A	117642	5.56	15%	54/51	12/43	−2	−2.3	SLTEYMQTVELKK,; MAETNNECSIKVLCR	Transport
418	P56536	Kinesin heavy chain isoform 5C (Fragment)	27376	5.87	25%	63/51	6/40	−2.7	−3.79	FVSSPEEVMDVIDEGK,; NRHVAVTNMNEHSSR	Transport
349	P48679	Lamin-A	74564	6.54	48%	221/56	32/68	−2.04	−2.28	LQDEMLRR,; LESSESR	Cytoskeleton
1263	Q6AYP2	Microfibrillar-associated protein 3-like	45804	4.9	13%	55/51	7/30	1	2.22	DEVYTIPNSLKR,; VTQFKTMEFAR	Sperm development
1355	P13832	Myosin regulatory light chain RLC-A	19940	4.67	62%	102/56	13/52	2.18	1.82	DGFIDKEDLHDMLASMGK,; GNFNYIEFTR	Muscle contraction
1357	Q64122	Myosin regulatory light polypeptide 9	19765	4.8	42%	68/51	10/48	2.12	1.5	EAFNMIDQNR,; KGNFNYVEFTR	Muscle contraction
1276	Q63716	Peroxiredoxin-1	22323	8.27	41%	77/56	7/44	−1.84	−2.56	ADEGISFR,; MSSGNAKIGHPAPSFK	Redox regulation
1213	P97562	Peroxisomal acyl-coenzyme A oxidase 2	77548	7.64	17%	53/51	10/41	2.19	1.96	HGMHAFIVPIR,; LAWSLGWSEDGPER	lipid metabolism
1198	P25113	Phosphoglycerate mutase 1	28928	6.67	40%	72/51	8/28	−1.66	−5.8	YADLTEDQLPSCESLKDTIAR,; VLIAAHGNSLR	Glycolysis
1202	P25113	Phosphoglycerate mutase 1	28928	6.67	60%	169/51	22/59	1.41	1.56	HGESAWNLENR,; FSGWYDADLSPAGHEEAK	Glycolysis
1405	P62963	Profilin-1	15119	8.46	75%	94/56	11/44	−3.01	1.12	EGVHGGLINK,; EGVHGGLINKK	Cytoskeleton regulation
1120	P18420	Proteasome subunit alpha type-1	29784	6.15	44%	115/56	12/28	−1.37	−1.53	NQYDNDVTVWSPQGR; QECLDSR	Protein degradation
1242	P40112	Proteasome subunit beta type-3	23235	6.15	43%	60/51	9/50	−3.09	−1.49	LNLYELKEGR,; NCVAIAADRR	Protein degradation
1300	P34067	Proteasome subunit beta type-4	29349	6.45	34%	70/56	12/62	6.23	6.45	FDCGVVIAADMLGSYGSLAR,; VNDSTMLGASGDYADFQYLK	Protein degradation
1321	P28075	Proteasome subunit beta type-5	28738	6.52	35%	75/56	8/48	15.04	13.13	GMGLSMGTMICGWDKR,; RGPGLYYVDSEGNR	Protein degradation
522	P11598	Protein disulfide-isomerase A3	57044	5.88	26%	77/56	11/38	1.31	1.69	GFPTIYFSPANK,; IFRDGEEAGAYDGPR	Redox regulation
1301	Q6IML7	Rab and DnaJ domain-containing protein	31329	8.72	26%	54/51	6/30	−3.01	−2.61	EPLKSLR,; CIDESEGRLWAESR	Signal transduction
781	P29315	Ribonuclease inhibitor	51653	4.67	57%	174/56	18/64	−1.56	−1.42	LSLQNCSLTEAGCGVLPDVLR,; LQLEYCNLTATSCEPLASVLR	Gene expression
952	P62138	Serine/threonine-protein phosphatase PP1-alpha catalytic subunit	38229	5.94	53%	163/56	16/49	−1.44	−1.52	TFTDCFNCLPIAAIVDEK,; IYGFYDECK	Signal transduction
397	P48721	Stress-70 protein (mitochondrial)	74097	5.97	33%	103/56	21/79	−2.12	−4.1	VCQGER,; DNMALQR	Chaperon
437	O35814	Stress-induced-phosphoprotein 1	63158	6.4	20%	88/51	12/62	1.26	1.56	AAALEFLNR,; TLLSDPTYR	Transport
1300	P83941	Transcription elongation factor B polypeptide 1	12636	4.74	45%	56/56	5/62	6.23	6.45	AMLSGPGQFAENETNEVNFR,; EIPSHVLSKVCMYFTYK	Gene expression
1208	Q91Y78	Ubiquitin carboxyl-terminal hydrolase isozyme L3	26278	5.01	63%	124/56	13/43	−1.64	−1.62	HLENYDAIR,; VDLHFIALVHVDGHLYELDGR	Protein degradation