MAPK Signal Transduction Pathway Regulation: A Novel Mechanism of Rat HSC-T6 Cell Apoptosis Induced by FUZHENGHUAYU Tablet
Figure 1
FUZHENGHUAYU Tablet induced apoptosis in rat HSC-T6 cells. Cultured rat HSC-T6 cells were divided into control and drug groups. The control group was incubated with DMEM medium containing 10% control serum, and the drug group was cultured with DMEM medium supplemented 10% drug serum for up to 72 h. Apoptosis was assessed using an Annexin V/7-AAD Apoptosis kit and analyzed using a BD FACS Calibur flow cytometer. (a) HSC-T6 cells exposed to 10% FBS and stained with Annexin V(+)/7-AAD(+); (b) HSC-T6 exposed to 10% FBS + 2 L/mL actinomycin D and stained with Annexin V(+)/7-AAD(+); (c) HSC-T6 cells exposed to 10% FBS + 2 L/mL actinomycin D and stained with Annexin V(+)/7-AAD(−); (d) HSC-T6 cells exposed to 10% FBS + 2 L/mL actinomycin D and stained with Annexin V(−)/7-AAD(+); (e) HSC-T6 cells exposed to 10% control serum and stained with Annexin V(+)/7-AAD(+). (f) HSC-T6 cells exposed to with 10% drug serum and stained with Annexin V(+)/7-AAD(+); (g) the late apoptotic cells in the drug (10% 1%) and control groups (5% 1%) (, *); (h) the early apoptotic cells in the drug (43% 6%) and control groups (26% 4%) (, *); (i) total apoptotic cells in the drug (53% 6%) and control groups (31% 4%) (, **).
