Evidence-Based Complementary and Alternative Medicine / 2013 / Article / Fig 2

Research Article

Pomegranate Bioactive Constituents Suppress Cell Proliferation and Induce Apoptosis in an Experimental Model of Hepatocellular Carcinoma: Role of Wnt/β-Catenin Signaling Pathway

Figure 2

Effects of PE on cyclin D1 expression during DENA-mediated hepatocarcinogenesis in rats. (A) Representative immunohistochemical localization of cyclin D1 (magnification: 100x). Arrows indicate immunohistochemical staining of cyclin D1. Several groups are (a) normal control; (b) DENA control; (c) PE 1 g/kg plus DENA; and (d) PE 10 g/kg plus DENA. (B) Quantification of cyclin D1-positive cells based on 1,000 hepatocytes per animal and 4 animals per group. Each bar represents the mean ± SEM ( ).   as compared with normal group;   as compared with DENA control. (C) Representative Western blot indicating protein expression levels of cyclin D1 in various experimental groups and (D) representative RT-PCR analysis of cyclin D1 expression in various groups of rats. Total hepatic RNA was isolated, subjected to reverse transcription, and resulting cDNA was subjected to RT-PCR analysis using specific primer sequence. The GAPDH was used as the housekeeping gene.