Pomegranate Bioactive Constituents Suppress Cell Proliferation and Induce Apoptosis in an Experimental Model of Hepatocellular Carcinoma: Role of Wnt/β-Catenin Signaling Pathway
Figure 4
Effects of PE on Bax and Bcl-2 expressions during DENA-mediated hepatocarcinogenesis in rats. (A) Immunohistochemical detection of Bax and Bcl-2 expression in the presence or absence of PE. (a)–(c) Representative immunohistochemical localization of Bax (magnification: 100x). Arrows indicate immunohistochemical staining of Bax. (d)–(f) Representative immunohistochemical localization of Bcl-2 (magnification: 100x). Arrows indicate immunohistochemical staining of Bcl-2. Several groups are ((a) and (d)) DENA control; ((b) and (e)) PE 1 g/kg plus DENA; and ((c) and (f)) PE 10 g/kg plus DENA. (B) Quantification of Bax-positive cells based on 1,000 hepatocytes per animal, and 4 animals per group. Each bar represents the mean ± SEM (). as compared with DENA control. (C) Quantification of Bcl-2-positive cells based on 1,000 hepatocytes per animal and 4 animals per group. Each bar represents the mean ± SEM (). as compared with DENA control. (D) Quantification of Bax/Bcl-2 ratio of positive cells based on 1,000 hepatocytes per animal and 4 animals per group. Each bar represents the mean ± SEM (). as compared with DENA control.
