Neurite Outgrowth in PC12 Cells Stimulated by Components from Dendranthema × grandiflorum cv. “Mottenohoka” Is Enhanced by Suppressing Phosphorylation of p38MAPK
Figure 8
Effects of various inhibitors on the level of phosphorylated ERK 1/2 stimulated by luteolin (B), acacetin (D), NGF, or EGF in PC12 cells. PC12 cells were cultured in serum-containing medium for 2 days. The cells were preincubated with K252a, AG1478, SB203580 (1 μM), or U0126 (10 μM) in serum-containing medium for 3 h. They were then treated or not treated with B, D, NGF, or EGF for 10 min. The level of phosphorylated ERK 1/2 of each sample was evaluated by immunoblotting. The intensities of each band of ERK 1/2 were measured densitometrically by Image J. Values are expressed as the ratios of phospho-ERK 1/2 to total-ERK 1/2, and the mean ± SEM of the values of three separate experiments are shown. Significant differences of the values from the value of the corresponding control group were determined by ANOVA, followed by Dunnett’s test. Control group is signified by . *: significance difference at ; **: significant difference at , —: no significant difference.
