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Evidence-Based Complementary and Alternative Medicine
Volume 2013, Article ID 412851, 12 pages
http://dx.doi.org/10.1155/2013/412851
Research Article

Peroxidase as the Major Protein Constituent in Areca Nut and Identification of Its Natural Substrates

1Department of Medical Research, China Medical University Hospital, Taichung 40402, Taiwan
2Department of Veterinary Medicine, National Chung Hsing University, Taichung 40227, Taiwan
3Graduate Institute of Integrated Medicine, China Medical University, Taichung 40402, Taiwan
4Proteomics Research Core Lab, China Medical University, Taichung 40402, Taiwan
5Department of Biochemistry, China Medical University, Taichung 40402, Taiwan
6Wholesaler Enterprise Company, Taipei 23941, Taiwan
7Department of Pharmacology, China Medical University, Taichung 40402, Taiwan
8Department of Biological Science and Technology, China Medical University, Taichung 40402, Taiwan
9Department of Biotechnology, Asia University, Taichung 41354, Taiwan

Received 6 June 2013; Accepted 4 September 2013

Academic Editor: Jen-Hwey Chiu

Copyright © 2013 Yu-Ching Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure S1. MS/MS spectra of C-C and EC-EC dimers. The dimerized ions at m/z 577 (A and B) and m/z 575 (D and E) are indicated. The MS/MS spectrum of dehydrodicatechin A in positive mode is also included (C).

Supplementary Figure S2. Inhibition of catechin and epicatechin oxidation by procyanidin B2. (A) and (B) Structures of procyanidins B1 and B2. (C) and (D) The inhibitory effect of procyanidin B2 on the oxidation of catechin (C) and epicatechin (D). The concentration of procyanidin B2 used is indicated.

Supplementary Figure S3. Zymogram detection of AN peroxidase in oral extracts. Peroxidases of the areca nut and Piper betle leaf are indicated by arrowheads. Lane 1, saliva alone as a control. Lane 2, areca nut coat. Lane 3, the intact areca nut. Lane 4, Piper betle leaf coated with slaked lime wrapped around the areca nut. Lane 5, Piper betle leaf coated with slaked lime.

  1. Supplementary Figures