An Engineered Arginase FC Protein Inhibits Tumor Growth In Vitro and In Vivo
Figure 3
rhArg-Fc inhibits tumor cell growth. (a) Three HCC cell lines (HepG2, Hep3B, and Huh7) and 1 melanoma cell line (SK-Mel-3) were incubated with rhArg-Fc at different concentrations for 3 days. Cell viability was determined as described previously [9]. (b) Three days after rhArg-Fc treatment, the cells were washed extensively with PBS and incubated further in fresh medium containing exogenous 1 mM L-arginine or L-glutamine for an additional 3 days. The number of cells was determined as described in Section 2.
