Propolis Modifies Collagen Types I and III Accumulation in the Matrix of Burnt Tissue
Figure 3
(a) Typical electropherogram of α (I) subunits, oligomers β/γ (I), and collagen type I degradation products, released from healthy and burnt skin. Collagenous components released from tissue samples using pepsin were submitted to 4–15% gradient SDS-PAGE, in nonreducing conditions. (b) Interferences of collagen type III, in electrophoretic profiles of collagen type I components extracted from healthy and burned skin. Collagen components were submitted to electrophoresis in the absence of dithiothreitol (reducing disulfide bonds) and subsequently—after electrotransfer to Immobilon—subjected to reaction with collagen type III antibodies. Lane 1: components of collagen type I isolated from healthy skin. Lane 2: components of collagen type I isolated from burned skin treated with propolis. Lane 3: components of collagen type I isolated from burned skin treated with AgSD. Lane 4: components of collagen type I isolated from burned skin treated with propolis vehicle. Lane 5: components of collagen type I isolated from burned skin treated with NaCl.
