4-Hydroxy-17-methylincisterol from Agaricus blazei Decreased Cytokine Production and Cell Proliferation in Human Peripheral Blood Mononuclear Cells via Inhibition of NF-AT and NF-𝜿B Activation

Figure 9

Effects of 4-HM on PBMC proliferation. PBMC (2×10⁵ cells/well) were treated with medium, DMSO (0.1%), indicated concentration of 4-HM, CsA (2 μM), or PDTC (20 μM) with or without PHA (5 μg/mL) for 3 days. The proliferation of cells was detected by ³H-thymidine uptake (1 μCi/well). After a 16 hr incubation, the cells were harvested by an automatic harvester, then radioactivity was measured by liquid scintillation counting. Each bar represents mean±SD from three independent experiments with PBMC from different individuals. ^∗𝑃<0.05, ^∗∗𝑃<0.01, ^∗∗∗𝑃<0.001: versus DMSO control.