4-Hydroxy-17-methylincisterol from Agaricus blazei Decreased Cytokine Production and Cell Proliferation in Human Peripheral Blood Mononuclear Cells via Inhibition of NF-AT and NF-𝜿B Activation

Table 1

The primer sequences used for amplification of cytokines and c-fos gene in PBMC.

Cytokine	Sequence	Predicted size (bp)
IL-2	5′: GTC ACA AAC AGT GCA CCT AC	262
	3′: GAA AGT GAA TTC TGG GTC CC
IL-4	5′: CGG CAA CTT TGA CCA CGG ACA CAA GTG CG	449
	3′: AGG ACA CTT CCT TCG GTT GGT CTC ATG CA
IFN-𝛾	5′: TCT GCA TCG TTT TGG GTT CTC	290
	3′: CTC TAC TGA AGC TTT TCG ACT
TNF-𝛼	5′: ACA AGC CTG TAG CCC ATG TT	428
	3′: TCA GAC CCG TCC AGA TGA AA
c-fos	5′: ATC AGC AGC ATG GAG CTG AAG ACC	606
	3′: CGT ACC TCA CAC ATA ACA AGG GTC
GAPDH	5′: TGA AGG TCG GAG TCA ACG GAT TTG GT	983
	3′: CAT GTG GGC CAT GAG GTC CAC CAC