4-Hydroxy-17-methylincisterol from Agaricus blazei Decreased Cytokine Production and Cell Proliferation in Human Peripheral Blood Mononuclear Cells via Inhibition of NF-AT and NF-𝜿B Activation

Table 2

Effects of 4-HM on cytokine gene expression in PBMC determined by real-time PCR.

	Relative cytokine mRNA levels (% referred to DMSO + PHA group)
	IL-2	IL-4	IFN-𝛾	TNF-𝛼
Medium	5.5±0.8	18.0±0.9	5.0±0.01	15.0±0.05
PHA	118±10	88.0±9.0	110±8.3	105±9.9
DMSO	5.2±0.03	18.0±1.8	4.8±0.03	16.8±0.03
DMSO + PHA	100±0.0	100±0.0	100±0.0	100±0.0
2.5 𝜇M 4-HM	4.9±0.02	15.0±0.09	4.2±0.005	17.5±0.08
2.5 𝜇M 4-HM + PHA	35.0±8.0^∗∗	36.0±3.4^∗∗	39.0±2.5^∗∗	51.0±6.8^∗∗
5.0 𝜇M 4-HM	5.0±0.01	6.8±0.09	5.1±0.006	17.0±0.5
5.0 𝜇M 4-HM + PHA	16.5±6.1^∗∗	18.0±3.6^∗∗	20.0±8.3^∗∗	35.0±5.5^∗∗
10 𝜇M 4-HM	5.0±0.6	5.2±0.04	5.4±0.4	14.0±2.6
10 𝜇M 4-HM + PHA	13.2±4.5^∗∗	12.4±3.3^∗∗	12.0±1.9^∗∗	23.0±5.2^∗∗

PBMC (5 × 10⁶) activated with or without PHA (5 𝜇g/mL) in the presence or absence of 4-HM (2.5, 5.0, 10 𝜇M) for 18 hr. The total cellular RNA was isolated from PBMC and aliquots of 1 𝜇g of RNA were reverse transcribed for synthesis of cDNA. Briefly, 10 𝜇L of cDNA was applied for the real-time PCR test as described in Section 2. ∗∗P < 0.01: versus DMSO + PHA group.