Inhibition of Acute Phase Inflammation by Laminaria japonica through Regulation of iNOS-NF-κB Pathway

Figure 7

Inhibition of LPS-induced iNOS and COX-2 expression by LJE. (a) Immunoblottings for iNOS and COX-2. iNOS or COX-2 protein levels were monitored 12 h after treatment with LPS (1 μg mL⁻¹) alone or in combination with LJE (3, 10, or 30 μg mL⁻¹). (b) Inhibition of LPS-induced iNOS expression and NO induction by LJE and isoliquiritigenin (ILQ). iNOS protein levels and NO concentration were monitored as described in legends of Figures 7(a) and 5(a), respectively. (c) Immunoblottings for phosphorylated I-κBα (p-I-κBα). The cells were treated with LPS or LPS + LJE for 30 min. The relative protein levels were measured by scanning densitometry. For a, b, and c, data represents the mean ± S.E.M. from three separate experiments (significant as compared with vehicle-treated control, ^∗𝑃<0.05, ^∗∗𝑃<0.01; significant as compared with LPS alone, ^#𝑃<0.05, ^##𝑃<0.01).

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