In Vitro Screening for Antihepatic Steatosis Active Components within Coptidis Rhizoma Alkaloids Extract Using Liver Cell Extraction with HPLC Analysis and a Free Fatty Acid-Induced Hepatic Steatosis HepG2 Cell Assay

Figure 4

The combinatorial analysis of seven alkaloids: 50.0 μg/mL CAE, 15.87 μg/mL of berberine, 5.32 μg/mL of coptisine, a high-affinity alkaloid group composed of 15.87 μg/mL berberine, 5.32 μg/mL coptisine, a low-affinity alkaloid group composed of 4.55 μg/mL palmatine, 2.28 μg/mL epiberberine, 0.55 μg/mL jateorhizine, 0.72 μg/mL columbamine, 0.65 μg/mL magnoline, and 100.0 μg/mL gemfibrozil. The selected concentration of alkaloids was based on the concentration ratio within 50.0 μg/mL CAE.