In Vitro Screening for Antihepatic Steatosis Active Components within Coptidis Rhizoma Alkaloids Extract Using Liver Cell Extraction with HPLC Analysis and a Free Fatty Acid-Induced Hepatic Steatosis HepG2 Cell Assay
Figure 5
The Oil Red O staining (400x) effect of combinatorial treatment: (a) control cells, (b) 0.5 mmol/L FFA-induced steatosis model cells, (c) 50.0 μg/mL CAE, (d) 15.87 μg/mL berberine, (e) 5.32 μg/mL coptisine, (f) a high-affinity alkaloid group composed of 15.87 μg/mL berberine and 5.32 μg/mL coptisine, (g) a low-affinity alkaloid group composed of 4.55 μg/mL palmatine, 2.28 μg/mL epiberberine, 0.55 μg/mL jateorhizine, 0.72 μg/mL columbamine, and 0.65 μg/mL magnoline, and (h) 100.0 μg/mL gemfibrozil.
