Ocotillol Enhanced the Antitumor Activity of Doxorubicin via p53-Dependent Apoptosis

Figure 3

The effects of ocotillol on the cell apoptosis induced by Dox in A549 cells. A549 cells were seeded into 6-well plate and treated with Dox (0.5 μM), Oco (0.5 μM), or the combination for 24 h. The cells were then subjected to flow cytometry assay (a) to determine the percentages of apoptotic cells (c) or to Hoechst staining assays (b). At least 1000 cells were randomly chosen, and the numbers of apoptosis cells (determined by the morphologic observation) were counted (d). ^∗𝑃<0.05, compared with control group; ^#𝑃<0.05, compared with Dox group.

(a)

(b)

(c)

(d)